Research
Interests
My interests are in
the physiology and ecology of microalgae, the
single-celled organisms at the base of the foodweb. Most of my work has dealt with trying to improve
estimates of their productivity, particularly in the
rapidly-changing conditions that are characteristic of
turbid estuaries. This
has involved studies of:
· regulation
of the enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase
(Rubisco), which plays a central role in the Calvin Cycle;
· investigation
of the ways in which varying environmental conditions of
nutrient availability, light history and temperature
affect the photosynthetic response;
· implementation
of bio-optical models of productivity;
· examination
of benthic-pelagic coupling as a driving force that favors
those microalgae associated with sediments vs those
suspended in the overlying water; and
· development
of a physiological/optical model of bloom development in
the Brown Tide pelagophyte Aureococcus
anophagefferens.
Another interest is in the use of
optical instruments for assessing the abundance,
productivity and taxonomic structure of the microalgae. This has involved use of absorption/scattering and
fluorescence as proxies for the pigment chlorophyll a,
which is frequently used as an index of microalgal
abundance by oceanographers. My lab’s field measurements are being used to
test and tune models based on satellite remote sensing, in
collaboration with Dr. Rick Gould (Naval Research
Laboratory) and Dr Greg Carter (
Gulf
Coast
Geospatial
Center
). I have also
worked on the use of variable fluorescence (PAM and FRRF)
as a proxy for carbon fixation, in collaboration with Drs
David Suggett and Richard Geider (
University of Essex
,
UK
) and Dr Todd Kana (
University
of
Maryland
). I am
currently funded, with Mr Richard Cox of Kaitech Inc. (
Columbia
MD
), to develop and test a laser-induced fluorescence sensor
that will identify the taxonomic structure of an
assemblage at about the class level. I hope that this will allow us to improve
bio-optical productivity models by introducing taxonomic
structure as a model input term. The instrument might also be used for water-quality
monitoring, for instance in real-time pre-screening of
water samples during harmful algal blooms (HABs) to
identify those most likely to contain the
potentially-toxic species. These would then be prioritized for testing, using
the appropriate microscopic or molecular techniques,
allowing more efficient use of human and laboratory
resources.
Courses
Offered
BLY430
Marine Botany (as a substitute for the regular
instructor Dr. Just Cebrian)
MAS 511 Analytical Methods
MAS 531 Physiological Ecology of Marine Microalgae
MAS 512 Chlorophyll Fluorescence Techniques – If you are
interested in taking this course (
May 14-25, 2007
), please contact me.
Current
Research Grants
US
EPA,
Alabama
Center
for Estuarine Studies -
Biomass, Taxonomic Distribution and Productivity of
Microalgae in
Mobile
and
Weeks
Bays
.
US
EPA - Environmental monitoring and primary production in
Mobile
Bay
:
A research and education initiative.
Alabama
Department of Conservation and Natural Resources - Little Lagoon as an Incubator Site for the
Harmful Bloom-Forming Diatom, Pseudo-nitzschia sp.
US
EPA,
Alabama
Center
for Estuarine Studies -
An Integrated Study on the Impacts of Watershed Changes on
the Turbidity and Biological Productivity in the
Mobile
Bay
Estuary,
AL
.
Alabama
Oyster Reef Restoration Program - Harmful algal blooms and oyster restoration in
Mobile
Bay
.
US
EPA,
Alabama
Center
for Estuarine Studies -
Impact of human activity on microalgal populations in
Mobile
Bay
:
Insights form recent palaeosediments.
Alabama
Department of Conservation and Natural Resources - Purchase and Testing
of an AutoLab Underway Nutrient Analyzer for Real-Time
Mapping during Harmful Algal Blooms
Centre
National de la Recherche Scientifique (France) - Photosynthetic Regulation and Ek-independent
Variability of Photosynthetic Response
NOAA
Cooperative Institute for Coastal and Estuarine
Environmental Technology - Improved Characterization of Microalgal Abundance
and Taxonomic Status through Laser-Induced Fluorescence (LIF)
Publications
Popels, L.C ., H.
L. MacIntyre, M. Warner, Y. Zhang and D. A. Hutchins. 2007. Physiological responses during dark survival
and recovery in Aureococcus anophagefferens (Pelagophyceae). J. Phycol in
press.
MacIntyre,
H. L. and J. J. Cullen. 2005. Using
cultures to investigate the physiological ecology of
microalgae. Pp. 287-326, In:
R. A. Anderson (Ed.), “Algal Culture
Techniques”. Elsevier
Academic Press,
Burlington
,
MA
.
Kana, T. M., M. W.
Lomas, H. L.
MacIntyre, J. C. Cornwell and C. J. Gobler.
2004. Stimulation of a brown tide organism, Aureococcus
anophagefferens, by selective nutrient additions to in
situ mesocosms. Harmful Algae 3:403-438.
Lomas, M. W., T. M.
Kana, H. L.
MacIntyre, and J. C. Cornwell. 2004. Interannual
variability of Aureococcus
anophagefferens in Quantuck Bay Long Island: Natural test of the DON hypothesis. Harmful
Algae 3:389-402.
MacIntyre,
H. L., M. W. Lomas, J. C. Cornwell, D. J. Suggett, E.
W. Koch, C. J. Gobler and T. M. Kana. 2004. Mediation
of benthic-pelagic coupling by microphytobenthos: An
energy- and material-based model for initiation of blooms
of Aureococcus
anophagefferens. Harmful Algae 3:403-438.
Pustizzi, F., H.
L.
MacIntyre
,
M. E.
Warner and D. A. Hutchins. 2004. Interaction
of nitrogen source and light intensity on the growth and
photosynthesis of the brown tide alga Aureococcus
anophagefferens. Harmful
Algae 3:343-360.
Suggett, D. J., H.
L. MacIntyre and R. J. Geider. 2004. Biophysical and optical determinations of
light absorption by photosystem II in phytoplankton.
Limnol. Oceanogr. Methods 2: 316-332.
Suggett,
D. J., K. Oxborough, N. Baker, H.
L. MacIntyre,
T. M. Kana and R. J. Geider. 2003. Fast
repetition rate and pulse amplitude modulation chlorophyll a fluorescence measurements for assessment of phytosynthetic
electron transport in marine phytoplankton. Eur. J.
Phycol. 38:371-384.
Geider,
R. J. and H.
L. MacIntyre.
2002. Physiology and biochemistry of photosynthesis
and algal carbon acquisition. Pp. 44-77, In:
P. J.
leB.
Williams, D. R. Thomas and C. S. Reynolds (Eds.),“Phytoplankton
Productivity and Carbon Assimilation in Marine and
Freshwater Ecosystems.” Blackwell Science,
Oxford
,
UK
.
MacIntyre,
H. L.,
T. M. Kana, T. Anning and R. J. Geider. 2002.
Photoacclimation of photosynthesis irradiance response
curves and photosynthetic pigments in microalgae and
cyanobacteria. J. Phycol. 38:17-38.
Anning,
T., H.
L. MacIntyre,
S. J. Pratt, P. Samms, S. Gibb and R. J. Geider.
2000. Photoacclimation in the marine diatom Skeletonema
costatum.
Limnol. Oceanogr. 45:1807-1817.
MacIntyre,
H. L.,
T. M. Kana and R. J. Geider. 2000. The effect of water
motion on short-term rates of photosynthesis by marine
phytoplankton. Trends in Plant Science 5:12-17.
Geider,
R. J., H.
L. MacIntyre,
L. M. Graziano and R. M. McKay. 1998.
Responses of the photosynthetic apparatus of Dunaliella
tertiolecta (Chlorophyceae) to nitrogen- and phosphorus-limitation.
Eur. J. Phycol. 33:315-332.
Geider,
R. J., H.
L. MacIntyre and
T. M. Kana. 1998. A dynamic regulatory model
of phytoplanktonic acclimation to light, nutrients and
temperature. Limnol. Oceanogr. 43:679 - 694.
Geider,
R. J., H.
L. MacIntyre and T. M. Kana. 1997. A dynamic model of
phytoplankton growth and acclimation: responses of the
balanced growth rate and the chlorophyll a:carbon
ratio of light, nutrient-limitation and temperature.
Mar. Ecol. Prog. Ser. 148:187-200.
MacIntyre,
H. L.,
T. D. Sharkey and R. J. Geider. 1997.
Activation and deactivation of ribulose-1,5-bisphosphate
carboxylase/oxygenase (Rubisco) in three marine microalgae.
Photosynth. Res. 51:93-106.
Geider,
R. J., H.
L. MacIntyre and T. M. Kana. 1996. A dynamic model of
photoadaptation in phytoplankton. Limnol. Oceanogr.
41:1-15.
MacIntyre,
H. L. and J. J. Cullen. 1996. Primary production by
suspended and benthic microalgae in a turbid estuary:
time-scales of variability in
San
Antonio Bay
,
Texas
.
Mar. Ecol. Prog. Ser. 145:245-268.
MacIntyre,
H. L. and
R. J. Geider. 1996. Regulation of Rubisco
activity and its potential effect on photosynthesis during
mixing in a turbid estuary. Mar. Ecol. Prog. Ser.
144: 247-264.
MacIntyre,
H. L.,
R. J. Geider and R. M. McKay. 1996.
Photosynthesis and regulation of Rubisco activity in net
phytoplankton from
Delaware
Bay
.
J. Phycol. 22:718-731.
MacIntyre,
H. L.,
R. J. Geider and D. C. Miller. 1996.
Microphytobenthos: the ecological role of the “secret
garden” of unvegetated shallow-water marine habitats.
I. Distribution, abundance and primary production.
Estuaries 19:186-201.
Miller,
D. C., R. J. Geider and H.
L. MacIntyre. 1996. Microphytobenthos: the ecological role of the
“secret garden” of unvegetated, shallow-water marine
habitats. II. Role in sediment stability and shallow
water food webs. Estuaries 19:202-212.
MacIntyre,
H. L. and
J. J. Cullen. 1995. Fine-scale vertical
resolution of chlorophyll and photosynthetic parameters in
shallow-water benthos. Mar. Ecol. Prog. Ser.
122:227-237.
Geider,
R. G., R. M. Greene, Z. Kolber, H.
L. MacIntyre and P. G. Falkowski. 1993. Fluorescence
assessment of the maximum quantum efficiency of
photosynthesis in the Western North Atlantic.
Deep
Sea
Res. 40:1205-1224.
Cullen,
J. J., X. Yang and H.
L. MacIntyre.
1992. Nutrient limitation and marine photosynthesis.
Pp. 69-88, In:
P. G. Falkowski and A. D. Woodhead (Eds.), “Primary
Productivity and Biogeochemical Cycles in the Sea”.
Plenum
Press
,
New
York
,
USA
.
Cullen, J. J., H.
L. MacIntyre and D. J. Carlson. 1989. Distributions
and photosynthesis of phototrophs in sea-surface films. Mar. Ecol. Prog. Ser. 55:271-278.
Cullen, J. J., C. M.
Yentsch, T. L. Cucci and H.
L. MacIntyre: 1988. Autofluorescence and other optical properties as
tools in biological oceanography. SPIE 925:149-156.
| Current
Graduate Students |
Post
Doctoral Associates |
Technicians |
Canion, Andy K. (M.S.)
Liefer, Justin (Ph.D.)
Novoveska, Lucie (Ph.D.)
Stapleton, Chuck (Ph.D.) |
|
Emily Goldman |
|
